New URI Genomics and Sequencing Center to open with NSF grantWhat's approximately the size of a mini-fridge and can unlock the secrets of DNA? The answer is a DNA sequencer, the centerpiece of URI's new Genomics and Sequencing Center (GSC), which opened in the Morrill Science Building in fall 2002. About the size of a campus dormitory fridge, the sequencer with associated equipment costs about $160,000 and was obtained with a grant from the National Science Foundation (NSF). This state-of-the-art unit reads the sequence of nucleotides that comprises DNA and determines individual hereditary characteristics. With this equipment in place, the GSC can provide high-quality DNA sequencing services to URI researchers in all departments and to external clients. "The establishment of the GSC will greatly enhance the university's focus on biotechnology, molecular biology, and genomics research," says David Nelson, URI microbiology professor and lead author on the NSF grant. "It also makes this exciting technology available to non-URI researchers at an affordable price." Soon to be added to the GSC is a real-time polymerase chain reaction (PCR) device, a $60,000 unit purchased with a U.S. Department of Agriculture grant arranged by Rhode Island's congressional delegation. PCR is a technique for amplifying DNA sequences and is best known to the public for its use as evidence in criminal law cases. PCR also has a wide range of applications in biotechnology, medicine, and genetic research. For example, by amplifying a specific DNA sequence, PCR can determine whether a pathogen or bioterror organism is present. Real-time PCR presents the reaction as it happens, providing results in minutes as opposed to hours or days. It enables investigators to rapidly and quantitatively determine whether a particular DNA or RNA sequence is present. For URI undergraduate and graduate students, the acquisition of the DNA sequencer and real-time PCR device offers an unparalleled opportunity to perform hands-on research using some of the most sophisticated technology now available. In addition to elevating the level and quality of their education, this experience provides students the specific training and skills required for high-paying careers in the biotechnology industry. Paul Johnson, manager of the GSC, handles the day-to-day operations of the center. A Web site for the GSC is under construction and can be reached at www.uri.edu/research/gsc for additional information about the facility, staff, sequencing protocols, and links to other molecular biology sites. —Arliss Ryan |